In this study, a comprehensive experimental design was employed to investigate the impact of four key factors on the spinning of polylactic acid (PLA) nanofibers. The four factors, namely the solute concentration of PLA, the solvent ratio, the spinning voltage, and the spinning receiving distance, were meticulously optimized through an L9(34) orthogonal experiment. The determination of the optimal process parameters for the preparation of PLA porous nanofiber membranes was achieved through the utilization of SEM and the analysis of fiber diameter data. The polymeric liquid adhesive was concentrated to 8 wt.%, the solvent ratio (DCM:DMF) was set at 9:1, the spinning voltage was set at 20 kV, and the spinning distance was set at 18 cm. In light of these findings, a series of PLA/GO porous nanofiber membranes with varying concentrations of graphene oxide (GO) were meticulously prepared. The study examined the impact of different GO contents on the proper-ties of the fibers. The experimental approach entailed the assessment of several parameters, including surface energy measurement and thermogravimetric analysis. The results demonstrated that when the GO concentration was set at 0.5 wt.%, the PLA/GO porous nanofiber membrane with uniform diameter distribution.